Journal de protéomique et d'enzymologie

Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis

Jun Cai, Jin Li, Changgao Wang, Jianguo Lin, Ying Hu, Jianhong Yang, Yumin Du and Hua Zheng

Parametric Optimization of Extracellular Chitin Deacetylase Production by Scopulariopsis brevicaulis

The culture medium, fermentation conditions and effects of different inorganic salts on producing extracellular chitin deacetylase by Scopulariopsis brevicaulis were optimized.The highest enzymatic activity of deacetylation of chitin was 36 units/mL for optimum culture medium which included 2% (w/v) 3,6-O-carboxymethylchitin, 1% sucrose, 0.2% NaNO3, 0.1% K2HPO4, 0.05% KCl, 0.4% peptone, 0.05% MgSO4, 0.05% MnSO4, 0.03% CoCl2 and optimum cultural conditions included temperature, pH, agitation speed, fermenting time and volume and were 29°C, 7.0, 200 rmp min-1, 96 hours and 80 mL in 300 mL Erlenmeyer flask, respectively