Journal des techniques de diagnostic et d'analyse biomédicale

Simplification of Molecular Methods

M. Giredhar1, Ram Singh2, B.S. Karotia2, A. Chakraborty3 and S. S.Lahiri2,3*

We have simplified and improved few techniques in furthering applications of molecular methods and products. This has been largely achieved by making them simple, ambient stable, rapid and cost effective. In this comprehensive article, beside simplification of few methods, we are reporting on devices like a “home-made cheap ultra-rapid thermal cycler” and a “homemade cheap and efficient Portable Transformation Device” for high efficiency transformation of one millilitre of fresh as well as stored cultures. Included in this paper are methods of extended storage of DNA at Room temperature in a ready-to-use form and for repeated utilization; development of ambient stable, rapid and cheapest in the world DNA Diagnostic kit for identification of micro-organisms; plant sex determination at nursery stage under Field as well as laboratory conditions. The simple kit can be used even by semiskilled personnel. Also reported here are sensitive and rapid detections from clinical samples; cheap and easy methods of DNA synthesis; easy, cheap, rapid and large scale DNA isolations. These methods can have wide applications in detection of infection and surveillance during outbreaks, pandemics, and as a routine exercise in the Border areas impending threat of Biological Warfare.