Journal de biologie vectorielle

Profile of The Internal Transcribed Spacer 2 in Ribosomal DNA of Tabanus rubidus in Thailand and Myanmar; The Possibility of Using for Molecular Identification

Apiwat Tawatsin, Payu Bhakdeenuan, Yang Chao , Tomoko Amano , Hitoshi Sasaki, Usavadee Thavara, Somchai Sangkitporn, Theeraphap Chareonviriyaphap and Padet Siriyasatien*

Tabanus rubidus is one of the most medically and veterinary important haematophagous insects. We demonstrated the profile of the Internal Transcribed Spacer 2 (ITS2) of ribosomal DNA in T. rubidus collected from various regions of Thailand and Myanmar. The ITS2 sequences in this study is high in A+T content (80.1%) and ranged from 589 to 622 bp. The highest intra and interspecific divergence among samples from same region was 1.2% and 11.2%, respectively. The sequences from this study were grouped by the same collecting region into individual clade using the Neighbor Joining with Kimura 2-Parameter tree. The data obtained in this study is useful for accurate species identification using ITS2 sequence which is the first step to provide the biological data for better integrated vector management programs for surra disease.